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Polink DS System

Polymer-HRP and Polymer-AP Double Staining System for Mouse & Rabbit

Polymer-HRP and Polymer-AP Double Staining System for 2 Mouse or 2 Rabbit 

 Polymer-HRP and Polymer-AP Double Staining System for Mouse & RabbitProduct Features

 

Product Features:

- Bio-Actin free double staining detection system

- Avoids  cross-reactions  sequential double staining                   

- Simultaneous incubation of primary antibodies

- Simultaneous incubation of secondary antibodies

- 4 Step method compared to sequential 14 step method

- Two formats:

     1. Polymer-HRP x Mouse and Polymer-AP x Rabbit

     2. Polymer-HRP x Rabbit and Polymer-AP x Mouse

 

 

Polink DS-MR kit is designed to simultaneously detect two different antigens at different locations

within the same tissue section. It utilizes proprietary high sensitivity polymer-HRP and polymer-AP

technology. The primary antibodies may be rabbit and mouse.

Comparing to conventional double staining methods, Polink DS-MR kit dramatically reduces

the staining steps from 14 steps to 4 steps. Polink DS-MR kit overcomes the cross-reactions

due to insufficient blocking of conventional sequential Biotin-Strepativdin/Alkaline Phosphatase

double staining method.

 

We offer two different formats for double staining:  Polymer-HRP x Mouse with polymer-AP x Rabbit

and polymer-HRP x Rabbit with polymer-AP x Mouse.  Refer to the following table to choose double

staining with brown color and red color (DAB + Fast Red) or red color and purple color (AEC + BCIP/NBT).

 

Product Name

Catalogue No

Enzyme conjugate & primary antibody

Chromogen

Data

Sheet

Polink DS-MR w DAB/AP-Red+

D20-6A

HRP x Mouse and AP x Rabbit

DAB & AP-Red+

Data Sheet

Polink DS-MR w DAB/Fast Red

D20-6F

HRP x Mouse and AP x Rabbit

DAB & Fast Red

Data Sheet

Polink DS-MR w BCIP/AEC

D63-6

HRP x Mouse and AP x Rabbit

AEC & BCIP/NBT

Data Sheet

Polink DS-MR w DAB/Fast Red

D32-6F

HRP x Rabbit and AP x Mouse

DAB & Fast Red

Data Sheet

Polink DS-MR w BCIP/AEC

D64-6

HRP x Rabbit and AP x Mouse

AEC & BCIP/NBT

Data Sheet

 

Polymer-HRP and Polymer-AP Double Staining System for 2 Mouse or 2 Rabbit

 

Product Features:

-Biotin-free

-Total of 7 steps compared to traditional 14 steps

-Can be used for double staining two antibodies from the same species

-Two formats:

     1. Polymer-HRP x Mouse and Polymer-AP x Mouse

     2. Polymer-HRP x Rabbit and Polymer-AP x Rabbit  

 Polink DS-MM kit and Polink DS-RR kit is designed to detect two different antigens at different locations

within the same tissue section. The primary antibodies may be two mouse antibodies or two rabbit antibodies.

It utilizes GBI’s proprietary DS Blocker to effectively prevent the second staining from interfering with the first

staining.  Compared to conventional double staining methods, Polink DS kit dramatically reduces the

staining steps from 14 steps to 7 steps. Polink DS kit overcomes the cross-reactions due to insufficient

blocking of sequential Biotin-Strepativdin/Alkaline Phosphatase double staining method.

 

We offer two different formats for double staining:  Polymer-HRP anti Mouse/polymer-AP anti Mouse

and polymer-HRP anti Rabbit/polymer-AP anti Rabbit.  Refer to the following table to choose double

staining with brown color and red color (DAB + Fast Red or AP-Red) or red color and purple color

(AEC + BCIP/NBT).

 

Product Name

Catalogue No

Enzyme conjugate & primary antibody

Chromogen

Data

Sheet

Polink DS-MM w DAB/Fast Red

D78-6F

HRP x Mouse and AP x Mouse

DAB & Fast Red

Data Sheet

Polink DS-MM w DAB/AP-Red+

D78-6A

HRP x Mouse and AP x Mouse

DAB & AP-Red+

Data Sheet

Polink DS-MM w BCIP/AEC

D79-6

HRP x Mouse and AP x Mouse

BCIP/NBT & AEC

Data Sheet

Polink DS-RR w DAB/Fast Red

D80-6F

HRP x Rabbit and AP x Rabbit

DAB & Fast Red

Data Sheet

Polink DS-RR w DAB/AP-Red+

D80-6A

HRP x Rabbit and AP x Rabbit

DAB & AP-Red+

Data Sheet

Polink DS-RR w BCIP/AEC

D81-6

HRP x Rabbit and AP x Rabbit

BCIP/NBT & AEC

Data Sheet

 

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