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Polymer-HRP and
Polymer-AP Double Staining System for Mouse & RabbitProduct
Features
Product Features:
- Bio-Actin free double staining detection
system
- Avoids cross-reactions
sequential double staining
- Simultaneous incubation of primary antibodies
- Simultaneous incubation of secondary antibodies
- 4 Step method compared to sequential 14 step method
- Two
formats:
1. Polymer-HRP x Mouse and Polymer-AP x Rabbit
2. Polymer-HRP x Rabbit and Polymer-AP x Mouse
Polink DS-MR kit
is designed to simultaneously detect two different
antigens at different locations
within the same tissue section.
It utilizes proprietary high sensitivity polymer-HRP and
polymer-AP
technology. The primary antibodies may be rabbit and
mouse.
Comparing to conventional double staining methods, Polink DS-MR
kit dramatically reduces
the staining steps from 14 steps to 4
steps. Polink DS-MR kit overcomes the cross-reactions
due to
insufficient blocking of conventional sequential Biotin-Strepativdin/Alkaline
Phosphatase
double staining method.
We
offer two different formats for double staining: Polymer-HRP x
Mouse with polymer-AP x Rabbit
and polymer-HRP x Rabbit with
polymer-AP x Mouse. Refer to the following table to choose
double
staining with brown color and red color (DAB + Fast Red)
or red color and purple color (AEC + BCIP/NBT).
|
Product Name |
Catalogue No |
Enzyme conjugate & primary antibody |
Chromogen |
Data
Sheet |
|
Polink DS-MR w
DAB/AP-Red+ |
D20-6A |
HRP x Mouse and
AP x Rabbit |
DAB & AP-Red+ |
Data Sheet |
|
Polink DS-MR w
DAB/Fast Red |
D20-6F |
HRP x Mouse and AP x Rabbit |
DAB & Fast Red |
Data Sheet |
|
Polink DS-MR w
BCIP/AEC |
D63-6 |
HRP x Mouse and AP x Rabbit |
AEC & BCIP/NBT |
Data Sheet |
|
Polink DS-MR w
DAB/Fast Red |
D32-6F |
HRP x Rabbit and AP x Mouse |
DAB & Fast Red |
Data Sheet |
|
Polink DS-MR w
BCIP/AEC |
D64-6 |
HRP x Rabbit and AP x Mouse |
AEC & BCIP/NBT |
Data Sheet |
Polymer-HRP and Polymer-AP Double Staining System for 2
Mouse or 2 Rabbit
Product Features:
-Biotin-free
-Total of 7 steps compared to traditional 14 steps
-Can be used for double staining two antibodies from the same
species
-Two
formats:
1. Polymer-HRP x Mouse and Polymer-AP x Mouse
2. Polymer-HRP x Rabbit and Polymer-AP x
Rabbit
Polink DS-MM kit and
Polink DS-RR kit is designed to detect two different
antigens at different locations
within the same tissue section.
The primary antibodies may be two mouse antibodies or two rabbit
antibodies.
It utilizes GBI’s proprietary DS Blocker to
effectively prevent the second staining from interfering with
the first
staining.
Compared to conventional double staining methods, Polink DS kit
dramatically reduces the
staining steps from 14 steps to 7
steps. Polink DS kit overcomes the cross-reactions due to
insufficient
blocking of sequential Biotin-Strepativdin/Alkaline
Phosphatase double staining method.
We
offer two different formats for double staining: Polymer-HRP
anti Mouse/polymer-AP anti Mouse
and polymer-HRP anti
Rabbit/polymer-AP anti Rabbit. Refer to the following table to
choose double
staining with brown color and red color (DAB +
Fast Red or AP-Red) or red color and purple color
(AEC + BCIP/NBT).
|
Product Name |
Catalogue No |
Enzyme conjugate & primary antibody |
Chromogen |
Data
Sheet |
|
Polink DS-MM w
DAB/Fast Red |
D78-6F |
HRP x Mouse and
AP x Mouse |
DAB & Fast Red |
Data Sheet |
|
Polink DS-MM w
DAB/AP-Red+ |
D78-6A |
HRP x Mouse and AP x Mouse |
DAB & AP-Red+ |
Data Sheet |
|
Polink DS-MM w
BCIP/AEC |
D79-6 |
HRP x Mouse and
AP x Mouse |
BCIP/NBT & AEC |
Data Sheet |
|
Polink DS-RR w
DAB/Fast Red |
D80-6F |
HRP x Rabbit and AP x Rabbit |
DAB & Fast Red |
Data Sheet |
|
Polink DS-RR w
DAB/AP-Red+ |
D80-6A |
HRP x Rabbit and AP x Rabbit |
DAB & AP-Red+ |
Data Sheet |
|
Polink DS-RR w
BCIP/AEC |
D81-6 |
HRP x Rabbit and AP x Rabbit |
BCIP/NBT & AEC |
Data Sheet |
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