Product Features:

- 2nd generation of polymer AP (non-biotin) system

- More sensitive than Polink-1 AP system with clean background

- 2-step polymer detection system

- Compatible with either manual or automated systems

- Better results on difficult-to-work antibodies and nuclear staining

- Save cost due to further dilution of primary antibodies

Polink-2 AP detection kit is the second generation polymer-based detection system that uses

proprietary polymer helper and polymeric alkaline phosphatase (AP)-linked secondary antibody

conjugates to achieve super sensitivity and consistent immunostaining.

This technology provides excellent sensitivity and high specificity.  It is about 3-4 times more

sensitive than the 1-step polymer-AP detection system and biotin-streptavidin system.   It helps to

achieve better results on difficult-to-work antibody and nuclear staining.  It is a biotin-free system,

so therefore overcomes the non-specific staining caused by streptavidin/biotin system due to

endogenous biotin.  Since it has higher sensitivity than other conventional methods and 1-step polymer

detection system (Polink-1 AP system), users may need to further dilute the concentration of primary

antibodies if switching from the conventional three-step or Polink-1 AP detection methods.  

Only broad spectrum detection kits are available in Polink-2 AP detection system.   For working with

single species or ultra sensitivity, please choose the third generation of polymer detection system,

Polink-2 Plus AP detection system, on page 20.

No chromogen is provided in the kit.  Refer to GBI Catalog page 29 to choose BCIP/NBT, Fast Red or

AP-Red+ to obtain desired color.

 

Product Name	Cat. No	Primary antibody	Chromogen	Data Sheet
Polink-2 AP Board Bulk Kit Polink-2 AP 15ml Kit Polink-2 AP 6ml Kit	D24-110 D24-15 D24-6	Mouse and Rabbit	Not Included	Data Sheet