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Trypsin
and Pepsin
Trypsin
Solution may be used
for enzyme digestion of formalin-fixed, paraffin-embedded
tissues, and will
improve the signal of
certain antigens. The kit contains 15 mL of Trypsin concentrate
and 80 mL of Diluent.
It requires simple
dilution prior apply on the tissue section.
Pepsin
Solution is used for the enzyme digestion of paraffin-embedded,
formalin-fixed tissues prior to staining
of certain antigens
by immunohistochemical methods. The pepsin solution is provided
in one single stabilized
ready-to-use
solution.
|
Product Name |
Catalogue number |
Size |
Data Sheet |
|
Trypin Solution |
E05-15 |
15ml Trypsin+80ml Diluent |
Data Sheet |
|
Pepsin Solution |
E06-50 |
50ml |
Data Sheet |
Blocking Solution
PreBlock
contains 10% normal goat serum. It is supplied in ready-to-use
format. It is used to block
non-specific binding
prior to primary antibody incubation.
Protein Block
is serum free
universal blocking reagent. It is used for reducing
non-specific background
in immunolabeling
procedures. It eliminates the handling of animal serum in the
laboratories.
Avidin/ Biotin Block
is used to
block endogenous biotin or biotin-binding activity in tissue
sections.
|
Product Name |
Catalogue number |
Size |
Data Sheet |
|
PreBlock Kit |
E07-100 / E07-15 |
100ml / 15ml |
Data Sheet |
|
Protein Block Kit |
E17-100 / E17-15 |
100ml / 15ml |
Data Sheet |
|
Avidin / Biotin
Block Kit |
E08-15 |
15ml each |
Data Sheet |
Other Retrieval Buffer
Citrate buffer and EDTA
solution is used in Heat Induced Epitope Retrieval (HIER) method
to recover the loss
of antigenicity that
sometimes occurs during fixation process. Choose a proper
buffer according to the
recommendations from the
primary antibody suppliers. Immerse slide in the proper buffer
heated by hot
plate (>95°C), or pressure
cooker. All buffer are supplied in concentrate format and
require simple dilution.

|
Product Name |
Catalogue No |
Size |
|
Citrate Buffer pH 4.0 (20x) |
B05C-100A |
100ml |
|
Citrate Buffer pH 6.0 (20x) |
B05C-100B |
100ml |
|
Citrate Buffer pH 7.1 (20x) |
B05C-100C |
100ml |
|
EDTA Solution PH 8.0 (20x) |
B04C-100 |
100ml |
|