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Technical Resource

 
 
 
 
 
 
Prestaining Reagents
 

 Trypsin                           

Trypsin may be used for enzyme digestion of formalin-fixed, paraffin-embedded tissues, and will improve the

signal of certain antigens. The kit contains 15 mL of trypsin concentrate and 80 mL of diluent. It requires simple

dilution prior apply on the tissue section.

 

Product Name

Catalogue number

Size

Trypsin

E05-15

15ml Trypsin + 80ml diluent

 

Pepsin                             

Pepsin is used for the enzyme digestion of paraffin-embedded, formalin-fixed tissues prior to staining of certain

antigens by immunohistochemical methods. The pepsin solution is provided in one single stabilized solution

and ready to use.

 

Product Name

Catalogue number

Size

Pepsin

E06-50

50ml

 

 

PreBlock (10% Normal Goat Serum)

This reagent is supplied in ready-to-use format. It is used prior to primary antibody incubation to block

non-specific binding.

                                          

Product Name

Catalogue number

Size

PreBlock (10% Normal Goat Serum)

E07-15

E07-100

15ml

100ml

 

Avidin/Biotin Block kit

Avidin/Biotin Block kit is used to block endogenous biotin or biotin-binding activity in tissue sections.

It contains 15 mL each of Avidin and Biotin solutions.

 

Product Name

Catalogue number

Size

Avidin/Biotin Block kit

E08-15

15 ml

 

Antibody Diluent          

Antibody Diluent is formulated to dilute concentrated primary antibodies for use in immunohistochemical

staining procedures. It is color coded ready-to-use reagent containing protein stabilizer and preservative.

 

Product Name

Catalogue number

Size

Antibody Diluent

E09-100

E09-500

100 ml

500ml

 

 

 

 

 

  

Isotype Control for mouse primary antibody              

This is a non-immunoglobulin that used as negative isotype control on the sample tissue in the same way as

mouse primary antibody to indicate if any non-specific staining occurs. The reagent is provided as 15ml

ready-to-use format.

  

Product Name

Catalogue number

Size

Good for slides

Isotype Control for mouse primary antibody

E10-15

15ml

150

 

 

Isotype Control for rabbit primary antibody                

This is a non-immunoglobulin that used as negative isotype control on the sample tissue in the same way

as rabbit primary antibody to indicate if any non-specific staining occurs. The reagent is provided as 15ml

ready-to-use format.

 

Product Name

Catalogue number

Size

Good for slides

Isotype Control for rabbit primary antibody

E11-15

15ml

150

                                

                                          

Base Buffer:                 

100mM PBS Reagent Grade            B01

 

0.1 M Tris buffer Reagent Grade      B02

 

Citrate buffer                   B03-1000

 

pH 8.0 EDTA                   B04-1000