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For over 50 years the Papanicolaou (PAP) Smear has been the Gold
Standard as a screening test for cervical cancer. The PAP
smears were prepared by collecting a cervical scraping with a
brush or other collection device and placed on a two slides,
fixed and allowed to dry. The slides were then processed
through a staining procedure. When the specimen was smeared
across the slide many thousands of cells are placed on each
slide making it impossible to obtain an even distribution of
cells. The preparation and staining of the slide was very
critical because of the uneven distribution of the cells. The
technologist reading the slide required a great deal of
competence because a positive slide would contain very few
cancerous or atypical cells. In the last decade researchers have
developed a thin layer procedure that would allow an even
distribution of the cells on the slide.
Mono-Solution TM is the Final Solution
There
were several types of automated thin layer slide preparation
procedures. These procedures allowed the even distribution of
the cells, much easier to read and gave better results than the
PAP smear. However, all automated procedures require not only
expensive equipment, but also high-cost of consumables (about 50
times higher than PAP smear). Many opinion leaders in this
field suggested carefully investigate the cost/effective factors
before switching PAP smear to an automated system. Cytologists
need a new method that can produce the same quality slides as
automated system with affordable price. To meeting the needs,
GBI has launched a new product, Mono-Solution TM.
Mono-Solution
TM is a single reagent that can be run at any cytology
laboratory or any clinic that has regular centrifuge.
Mono-Solution TM
helps to remove unwanted components in the sample, such as mucus
or blood, therefore, reduces sample error. It ensures to
produce evenly distributed thin layer or mono-layer cells on the
slide. Mono-SolutionTM requires a centrifugation
steps to avoid negative dilution effect. This allows a greater
detection of abnormal cells. Although the Mono-SolutionTM
is not an automated procedure, it can be completed at a fraction
of the cost with superior results. The Mono-Solution TM
requires 4 milliliters of one reagent in a Collection tube. It
can be supplied in tubes or bulk.
EASY APPLICATION
1.
Add 4 ml Mono-SolutionTM
buffer to the tube. Label the tube properly. Dip the sample
collector (cytology brush or scraper) in the tube and stirs
about 20 seconds. Make sure sample cells are eluted in the
buffer. This step is very crucial for getting as many cells on
the slide.
2. Place specimen
tube in centrifuge and spin at 1,200 rpm for not less than 5
minutes.
3. Remove tube and
use aspirator or disposable pipette to remove supernatant and
leave as many cell component as possible.
4. Vortex or agitate
vigorously for 5-10 seconds, to create a homogeneous slurry.
5. Use disposable
pipette to place 3- 4 drops of the slurry on each of two slides
per (A): total 6-8 drops.
6. Place on slide on
top of the other per (B and (C).
7. Pull slides apart
per D.
8. Allow Slides to
dry
9. Stain per standard
cytology protocol (DO NOT NEED FIXATION). And read
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