The Polin-2 Plus HRP or AP Detection systems is considered to be the best polymer detection system on the market. Comparing the existing systems from other suppliers, our Poin-2 Plus system is ultra sensitive with clean background. our staining performance is 2-4 times stronger without a noticeable increase in background noise...more

Immunohistochemistry, or IHC, refers to the process of detecting proteins expressed in cells of a tissue section using antigens/antibodies interaction to visualize localization of expressed proteins. Immunohistochemical staining is widely used in pathology labs and basic research to understand the distribution and localization of biomarkers in different parts of a tissue.  Current immunohistochemistry detection systems include Peroxisae-anti-Peroxidase (PAP), the Avidin-Biotin Complex (ABC), the Biotin-Streptavidin Amplified   (B-SA) systems, and the polymer-enzyme system.

IHC Detection System is a proprietary technology of Golden Bridge International (GBI). It includes the improved Biotin-Streptavidin amplification system which includes SPlink and APlink systems, and the Polymeric amplification system, which includes the Polink-1, Polink-2, and Polink-2 Plus systems.

SPlink (Streptavidin-Peroxidase) and APlink (Streptavidin-Alk. Phosphatase)  detection kits are supplied in ready-to-use format. In this amplification system, the secondary antibody is conjugated with Biotin to its maximum extent with high binding affinity. This system not only demonstrate superior sensitivity with a super clean background, but it also provides excellent staining stability with a longer shelf life (storage period) because of an improved stabilizer formula.  A shorter incubation period (10’/10’ minutes for 2^nd Antibody and enzyme conjugate) provides customers with faster results.

Polink-1 detection system is based on a novel polymeric technology. In this technology, the second antibody was polymeric conjugated with peroxidase or alkaline-phosphatase by using a synthetic polymer carrier. This approach increases the number of available enzymes, or ligands, binding at the antigenic site, thus increasing their reactivity to chromogen.  It is a biotin-free system that eliminates non-specific binding due to endogenous biotin.  It provides higher sensitivity, detection efficiency, simpler protocol, easier standardization, and less procedural variability.

Polink-2 detection system is the second generation of GBI’s polymer detection system that uses a proprietary polymer helper and polymeric HRP-linked or AP-linked secondary antibody conjugates to achieve higher sensitivity than the Polink-1 system with only one extra step.  It can achieve equivalent immunostaining at a much higher dilution of primary antibodies than the first generation of polymer detection (Polink-1) system, especially on difficult-to-work antibodies.  Polink-2 detection system offers laboratories the advantage of significant higher amplification of staining intensity yet lower costs resulting from more dilution of primary antibodies.   It eliminates potential background due to endogenous biotin activity.